axiovert200m epifluorescent microscope  (Carl Zeiss)

Journal: Frontiers in Molecular Neuroscience

Article Title: The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System

doi: 10.3389/fnmol.2017.00230

Figure Lengend Snippet: Semi-thin (0.5 μm-thin) sections of the mouse cerebellum double-immunolabeled with the monoclonal dynamin1xb antibody and the indicated other primary antibodies. The other primary antibodies against synaptotagmin1 (A,B) , synaptic vesicle protein 2 (SV2; C ) and RIM1/2 (D) were applied to label the synapses in order to better relate the dynamin1xb immunosignals to the synaptic regions. We observed a strong dynamin1xb immunosignal in the cerebellar cortex whereas the cerebellar medulla (white matter) that contains predominantly fiber tracts (but no synapses) was not immunolabeled. In the cerebellar cortex, dynamin1xb was highly enriched in the synaptic regions, i.e., the molecular layer (mol) of the cerebellar cortex and the giant synapses in the granule cell layer (arrows) of the cerebellar cortex. No significant dynamin1xb immunosignal was observed in the medulla of the cerebellum that predominantly contains axonal fiber tracts. (A,B,D) was obtained by epifluorescence microscopy; (C) was obtained by confocal microscopy. Abbreviations: mol, molecular layer; Pu, Purkinje cell layer; gr, granule cell layer. Scale bars: 50 μm (A–D) .

Article Snippet: From the immunolabeled sections, images were acquired either with a Zeiss epifluorescence microscope setup (Axiovert200M) equipped with the respective filter blocks or with a Nikon A1R confocal microscope, as indicated in the respective experiments.

Techniques: Immunolabeling, Epifluorescence Microscopy, Confocal Microscopy

Journal: Frontiers in Molecular Neuroscience

Article Title: The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System

doi: 10.3389/fnmol.2017.00230

Figure Lengend Snippet: Semi-thin (0.5 μm-thin) sections of the mouse spinal cord (A–C) and the mouse visual cortex (D) double-immunolabeled with the monoclonal antibody against dynamin1xb and the indicated other primary antibodies. The rabbit polyclonal antibodies against synaptotagmin1 (B,D) and RIM1/2 (A) were applied to label the synapses in the spinal cord. Immunolabeling with rabbit polyclonal antibodies against β-tubulin was used to also visualize the neuronal axons in the white matter of the spinal cord (C) . We observed a strong dynamin1xb immunosignal in the gray matter of the spinal cord whereas the white matter that contains many axons (but virtually no synapses) was largely unlabeled by the dynamin1xb antibody. High-resolution confocal analyses revealed the presence of dynamin1xb in synatotagmin1- labeled presynaptic terminals that contact the cell bodies of motor neurons in the gray matter of the spinal cord (B) . Similarly, also in the visual cortex (D) , we observed a dynamin1xb immunolabeling signal that largely overlapped with synapses as judged by anti-synaptotagmin1 immunolabeling. Arrow in (C) points to an exemplary axon in the white matter of the spinal cord that was immunolabeled with anti-β-tubulin antibodies. (A,C) were obtained by epifluorescence microscopy; (B,D) by confocal microscopy. Abbreviations: n, nucleus of a motor neuron in the anterior horn of the spinal cord; mol, molecular layer; e-gran, external granule cell layer. Scale bars: 50 μm (A,C) ; 10 μm (B) ; 30 μm (D) .

Article Snippet: From the immunolabeled sections, images were acquired either with a Zeiss epifluorescence microscope setup (Axiovert200M) equipped with the respective filter blocks or with a Nikon A1R confocal microscope, as indicated in the respective experiments.

Techniques: Immunolabeling, Labeling, Epifluorescence Microscopy, Confocal Microscopy